grna expression cassette Search Results


grna expression cassette  (GenScript corporation)
90
GenScript corporation grna expression cassette
Grna Expression Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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grna expression cassette - by Bioz Stars, 2026-05
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u6 promoter:grna expression cassettes  (GenScript corporation)
90
GenScript corporation u6 promoter:grna expression cassettes
U6 Promoter:Grna Expression Cassettes, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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u6 promoter:grna expression cassettes - by Bioz Stars, 2026-05
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grna expression cassettes  (Twist Bioscience)
90
Twist Bioscience grna expression cassettes
Grna Expression Cassettes, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequence of three grna expression cassettes  (Oligos Etc)
90
Oligos Etc sequence of three grna expression cassettes
Sequence Of Three Grna Expression Cassettes, supplied by Oligos Etc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequence of three grna expression cassettes - by Bioz Stars, 2026-05
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pgcas9 -expressing cassette and u6 promoter: grna backbone  (GenScript corporation)
90
GenScript corporation pgcas9 -expressing cassette and u6 promoter: grna backbone
The T-DNA structure <t>of</t> <t>PgCas9</t> / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic <t>tRNA-gRNA</t> (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.
Pgcas9 Expressing Cassette And U6 Promoter: Grna Backbone, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgcas9 -expressing cassette and u6 promoter: grna backbone/product/GenScript corporation
Average 90 stars, based on 1 article reviews
pgcas9 -expressing cassette and u6 promoter: grna backbone - by Bioz Stars, 2026-05
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cassette for polycistronic grna expression  (GenScript corporation)
90
GenScript corporation cassette for polycistronic grna expression
The T-DNA structure <t>of</t> <t>PgCas9</t> / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic <t>tRNA-gRNA</t> (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.
Cassette For Polycistronic Grna Expression, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cassette for polycistronic grna expression/product/GenScript corporation
Average 90 stars, based on 1 article reviews
cassette for polycistronic grna expression - by Bioz Stars, 2026-05
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dox-inducible single grna expression cassette  (Aubrey Inc)
90
Aubrey Inc dox-inducible single grna expression cassette
The T-DNA structure <t>of</t> <t>PgCas9</t> / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic <t>tRNA-gRNA</t> (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.
Dox Inducible Single Grna Expression Cassette, supplied by Aubrey Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dox-inducible single grna expression cassette/product/Aubrey Inc
Average 90 stars, based on 1 article reviews
dox-inducible single grna expression cassette - by Bioz Stars, 2026-05
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grna expression cassette promoter u6.6 medicago truncatula  (Medicago)
90
Medicago grna expression cassette promoter u6.6 medicago truncatula
The T-DNA structure <t>of</t> <t>PgCas9</t> / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic <t>tRNA-gRNA</t> (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.
Grna Expression Cassette Promoter U6.6 Medicago Truncatula, supplied by Medicago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
grna expression cassette promoter u6.6 medicago truncatula - by Bioz Stars, 2026-05
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trc promoter-driven grna expression cassette  (General Biosystems Inc)
90
General Biosystems Inc trc promoter-driven grna expression cassette
The T-DNA structure <t>of</t> <t>PgCas9</t> / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic <t>tRNA-gRNA</t> (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.
Trc Promoter Driven Grna Expression Cassette, supplied by General Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trc promoter-driven grna expression cassette/product/General Biosystems Inc
Average 90 stars, based on 1 article reviews
trc promoter-driven grna expression cassette - by Bioz Stars, 2026-05
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Image Search Results


The T-DNA structure of PgCas9 / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic tRNA-gRNA (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.

Journal: Frontiers in Plant Science

Article Title: Efficient Multi-Sites Genome Editing and Plant Regeneration via Somatic Embryogenesis in Picea glauca

doi: 10.3389/fpls.2021.751891

Figure Lengend Snippet: The T-DNA structure of PgCas9 / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic tRNA-gRNA (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.

Article Snippet: The final sequence containing PgCas9 -expressing cassette and U6 promoter: gRNA backbone was synthesized by Genscript Biotechnology Co., Ltd (Nanjing, China) and ligated into the modified pCAMBIA1300 vector, in which the Bsa I site was removed with QuickMutation TM (D0206, Beyotime Biotechnology, Shanghai, China), following the manufacturer's introduction.

Techniques: Marker, Expressing, Plasmid Preparation